Composite

Part:BBa_K4229077:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-10-09)


SPD5 with snoopTag&spyTag +RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2405
    Illegal EcoRI site found at 3579
    Illegal SpeI site found at 1383
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2405
    Illegal EcoRI site found at 3579
    Illegal SpeI site found at 1383
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2405
    Illegal EcoRI site found at 3579
    Illegal BglII site found at 2306
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2405
    Illegal EcoRI site found at 3579
    Illegal SpeI site found at 1383
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2405
    Illegal EcoRI site found at 3579
    Illegal SpeI site found at 1383
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Part:BBa_K3009033

References

C. D. Reinkemeier, G. E. Girona, and E. A. Lemke, “Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes,” Science (80-. )., vol. 363, no. 6434, 2019, doi: 10.1126/science.aaw2644.